1. <tbody id="wq0no"></tbody>
        <span id="wq0no"></span>
        1. <th id="wq0no"></th>
          <th id="wq0no"></th>

        2.  
          邱錄貴課題組與常洪課題組合作在Clinical Cancer Research期刊發表文章

            我所邱錄貴課題組與常洪課題組合作在Clinical Cancer Research期刊發表文章“ miR-137 and miR-197 Induce Apoptosis and Suppress Tumorigenicity by Targeting MCL-1 in Multiple Myeloma ”。

          Abstract

            

          PURPOSE:

            Deregulation of miRNA has been implicated in the pathogenesis of multiple myeloma. We identified miR-137 and miR-197, mapped to the chromosome 1p (12)-(21) deletion region, and examined their antimyeloma activity as tumor suppressors.

          EXPERIMENTAL DESIGN:

            The expression of miR-137/197 was examined in multiple myeloma and normal plasma cells by qRT-PCR. Functional effect of miR-137/197 was analyzed by cell viability, apoptosis, clonogenic, and migration assays. Antimyeloma activity of miR-137/197 was further evaluated in vivo by lentiviral-based or lipid-based delivery in a mouse xenograft model of multiple myeloma.

          RESULTS:

            miR-137/197 expression was significantly lower in multiple myeloma cell lines and multiple myeloma patient samples compared with normal plasma cells. Transfection of miR-137/197 resulted in reduction of MCL-1 protein expression, as well as alteration of apoptosis-related genes, and induction of apoptosis, inhibition of viability, colony formation, and migration in multiple myeloma cells. MCL-1 was further validated as a direct target of miR-137/197. Conversely, overexpression of MCL-1 partially reverted the effect of miR-137/197. Importantly, in vivo lentiviral-mediated or intratumor delivery of miR-137/197 induced regression of tumors in murine xenograft models of multiple myeloma.

          CONCLUSIONS:

            Our study reveals a novel role of miR-137/197 as tumor suppressors in mediating apoptosis in multiple myeloma cells by targeting MCL-1. Our findings provide a proof-of-principle that lentivirus-based or formulated synthetic miR-137/197 exerts therapeutic activity in preclinical models, and support a framework for development of miR-137/197-based treatment strategies in patients with multiple myeloma.

            ©2015 American Association for Cancer Research.

          查閱原文鏈接

             
          網站導航 | 聯系我們 | 使用幫助 | 網站聲明


          主辦:中國醫學科學院血液學研究所血液病醫院  技術支持:北方網
            備案序號:津ICP備05001070號
          建議使用IE6.0以上瀏覽器,分辨率1024*768

          野狼舍区华人第一社区